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. 2017 May 1;8:498. doi: 10.3389/fimmu.2017.00498

Figure 3.

Figure 3

Infectious, hematological and iron metabolism parameters in wild-type (WT) and hepcidin knockout (Hamp−/−) mice following infection with Salmonella Typhimurium (ST). Blood parameters from WT and hepcidin knockout Hamp−/− including hematocrit (A), iron (B), and ferritin (C) were analyzed before (−) or after 10 days of infection with ST (+ST). Iron levels were assessed by Perls staining (D,G) and dosage (E) on liver (D,E) or splenic (G) tissues. Bacterial load at day 10 of infection with ST was studied by CFUs in the liver (F) and spleen (H) of infected WT and Hamp−/− mice. Statistical significance: ***P < 0.001; ****P < 0.0001. (I) Hepatic iron localization in WT and Hamp−/− mice before and after Salmonella infection. Perls staining of liver sections from naive (−) mice or mice infected with ST (+ST, Day 10). Lower panels in WT mice show high magnification of the cellular localization of iron in Kupffer cells. PP, periportal vessels; CL, centrilobular vessels. n = 4–15 mice per genotype. The data are presented as mean ± SD.