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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Cell Rep. 2015 Oct 29;13(6):1221–1232. doi: 10.1016/j.celrep.2015.09.074

Figure 1. Identification of the pre-axonal exclusion zone.

Figure 1

(A) Z-stack reconstruction of confocal sections from DIV10 hippocampal neurons stained for endogenous TfR (red) and AnkG (green). (B, C) Magnified views of axons emanating from the soma (B) or a dendrite (C), and their neighboring dendrites. Graphs on the right show the corresponding TfR and AnkG intensity scans over a 10-μm line running from the soma to the axons or dendrites. In A–C, arrows point to the pre-axonal exclusion zone (PAEZ). (D) Images of axons emanating from the soma in neurons stained as in panel A at different times in culture. The PAEZ is the area delimited by dashed lines (images on the right). The top right picture marked with an asterisk is a higher intensity image of the top left image. (E) Graph representing the AIS area (green), PAEZ area (gray) and dendrite/axon (D/A) polarity index (red bars), calculated from 25 neurons at different days of culture in vitro. D/A values are the mean ± SD. (F) General view of the same DIV3 neuron shown in the top panels of D co-stained with phalloidin (grayscale, bottom) to highlight F-actin in the entire cytoplasm.