Inhibition
of colony formation on the DAP-Cys-TEG-NPrSi-O-Ti6Al4V
surfaces. The foils were incubated at 37 °C. Colonization of
bacteria on the surface of Ti6Al4V foils was recorded after 2, 4,
8, and 24 h incubation. Following incubation, the foils were washed
seven times with PBS, then stained for 20 min at rt with a live/dead
BacLight kit to visualize live bacteria by green fluorescence, with
nonmodified Ti6Al4V foils as the controls. Three independent sets
of foils were analyzed. Representative fluorescence surface plots
of colonies, processed to appear as 3D colorized peaks, are shown
at 40× magnification.