Figure 6.

The acidification of platelets secretory granules and the release of granule constituents during adenosine diphosphate (ADP)-induced activation in the presence of platelet aggregation inhibitor from the Echis multisquamatis (PAIEM) snake venom. Platelets were loaded with pH-sensitive fluorescent dye acridine orange and stimulated with 2 μM of ADP in the presence or absence of PAIEM. Traces are typical for 5 independent experiments. A – after the addition of equivalent amount of buffer; B – in the presence of 10 μg/mL of PAIEM; C – in the presence of 1% dimethyl sulfoxide; D – summarized enlarged fragment of graphics A-C. Traces are typical for 3 independent experiments performed 5 times each.