Table 1.

Comparison of deterministic SPADE and manual gating, expressed in terms of the number of overlapping cells between each manual gate and each annotated SPADE tree region.

		Manual gates
		B cell – 150,314	T cell – 14,699	CD4+ – 2,808	CD8+ – 6,055	Myeloid – 209,079	HSPC – 418
Annotated deterministic SPADE	B cell – 150,375	144,616 (96.2%)	8 (<0.1%)	0	0	2,286 (1.1%)	0
	Dendritic – 6,517	5,687 (3.8%)	0	0	0	282 (0.1%)	0
	T cell – 17,158	0	12,796 (87.1%)	2,806 (99.9%)	6,047 (99.9%)	2,435 (1.2%)	0
	CD4+ – 2,964	0	2,897 (19.7%)	2,791 (99.4%)	0	22 (<0.1%)	0
	CD8+ – 7,753	0	7,478 (50.9%)	0 6,040	(99.8%)	36 (<0.1%)	0
	Myeloid – 204,687	0	87 (0.6%)	0	0	202,300 (96.8%)	0
	c-kit+ – 17,924	7 (<0.1%)	852 (5.8%)	2 (<0.1%)	8 (<0.1%)	1,725 (0.8%)	402 (96.2%)

The total number of cells in each gate and each annotated region are listed. The percentages are defined as the ratio between the number of overlapping cells and the total number of cells in the corresponding manual gate, thereby representing the percent of cells in a gate that are assigned to each SPADE region. Large values in shaded entries indicate the consistency between manual gating and deterministic SPADE.