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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 13;1833(8):1992–2003. doi: 10.1016/j.bbamcr.2013.02.033

Figure 4. Blockade of the mPTP opening, but not inhibition of caspases, prevents HSCs death induced by nilotinib.

Figure 4

Effects of Z-VAD-FMK (Z-VAD, 50 μM) and cyclosporin A (CSA,20 μM) on nilotinib (20 μM)-induced apoptosis as evaluated under the phase contrast microscope (A) and by flow cytometric analysis using AV-FITC (FL1-H) and PI (FL2-H) staining (B–C), and the protein expression of PARP/cleaved PARP (D–E) as assessed by Western blotting for the total lysates of LX-2 cells. Data are means ± SE of at least 4 independent experiments. Statistically significant differences are indicated as: *P<0.05 and ***P<0.001 versus CTRL; ∇∇∇P<0.001 versus nilotinib (20 μM).