Fig. 3.

Potentiation of ASIC3-containing heteromers by RPRFa. (A) Representative current traces of heteromeric ASIC1a/3, ASIC1b/3, ASIC2a/3, and ASIC2b/3, activated with pH 6.3, 6.0, 5.7, and 6.5, respectively. Roman numerals mark specific activations. RPRFa (50 μM; red bars) was preapplied at pH 7.4 for 60 s. (B) Quantification of peak currents. Peak current amplitudes of the second activation with RFa preapplication were normalized to the first activation without RFa preapplication. (C) Quantification of current amplitudes 10 s after activation, normalized to the peak current amplitude (n = 10–18). *P < 0.05; ***P < 0.001 (Student’s t test).