Fig. 5.
RPRFa strongly potentiates ASIC3 when applied in the closed state. (A) Representative current trace of ASIC3 repeatedly activated with pH 6.3 (black bars). Roman numerals mark specific activations. RPRFa (10 μM) was applied at different times, as indicated by red bars. (B) Quantification of currents 10 s after activation, normalized to the respective peak currents. (C) Quantification of ai (n =10); significance vs. the first control activation i is indicated. *P < 0.05; ***P < 0.001 (one-way repeated-measures ANOVA). (D) Kinetic scheme illustrating basic states during gating of ASIC3.