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. 2017 Apr 10;114(17):E3507–E3515. doi: 10.1073/pnas.1616232114

Fig. 7.

Fig. 7.

RPRFa potentiates acid-induced transient muscle pain and chronic hyperalgesia mediated by ASIC3. (A) Enhancing effect of RPRFa on acid-induced mechanical hyperalgesia (n = 8 for each group). Transient and long-lasting hyperalgesia was only observed after the first injection of pH 6.5 saline with RPRFa (day 0) and after the second injection of acidic saline (pH 4.0) (day 2), respectively (n = 8 for each group). ***P < 0.001, RPRFa vs. RPRF; ###, P < 0.001, RPRFa vs. vehicle; ##, P < 0.01, RPRFa vs. vehicle (two-way repeated-measures ANOVA). (B) Injection of RPRFa (n = 5), RPRF (n = 6), or vehicle (n = 4) with neutral pH 7.4 saline showed no significant potentiation of transient or long-lasing hyperalgesia. (C) Asic3−/− mice did not show an increased response to mechanical stimuli after coinjection of RPRFa and pH 4.0 acidic saline (n = 6). Black arrows indicate the first intramuscular injection of saline (pH 6.5, 7.4, or 4.0, respectively) with or without RPRFa/RPRF; red arrows indicate the second intramuscular injection of acidic saline (pH 4.0). B, baseline on day 0.