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. 2017 Apr 10;114(17):4525–4530. doi: 10.1073/pnas.1619700114

Fig. S3.

Fig. S3.

Effects of GAD65LH cell stimulation on eating and body weight. (A) Scheme for targeting the excitatory CNO receptor hM3Dq (or control protein ChR2-YFP) to GAD65LH cells. Targeting was confirmed by histology, as shown in Fig. 3E. (B) Effect of CNO on food intake and body weight in GAD65-Ires-Cre mice expressing ChR2 or hM3Dq in GAD65LH cells (five mice per group). Values are mean ± SEM. CNO was injected daily (0.5 mg/kg i.p.) during the indicated days. (C) Data from B, analyzed with two-way repeated measures ANOVA. pre, day 5; CNO, day 9. (Top) Interaction [F(1,8) = 0.01474, P = 0.9064]. ns, not significant. (Bottom) Interaction [F(1,8) = 13.47, P = 0.0063; numbers above bars are P values from Tukey’s multiple comparison test].