Fig. 2.

EntV inhibition of hyphal morphogenesis and biofilm formation in C. albicans. (A) C. albicans SC5314 biofilms grown for 24 h in YNBAS with sterile media (M9HY) or spent supernatant from wild-type and mutant E. faecalis strains. Hyphal morphogenesis was quantified by measuring GFP fluorescence as a ratio to the OD₆₀₀. The experiment was performed three times and analyzed by using one-way ANOVA (**P < 0.01). (B) Biofilms were grown with increasing concentrations of rEntV¹³⁶ or sEntV⁶⁸ and the IC₅₀ calculated at 1,000 nM and 0.3 nM, respectively. (C) C. albicans was grown at 30 °C in YPD for 15 h in a 96-well plate with increasing concentrations of sEntV⁶⁸, and OD₆₀₀ was measured every 2 min in a microplate reader.