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. 2017 Apr 10;114(17):4507–4512. doi: 10.1073/pnas.1620432114

Fig. 5.

Fig. 5.

Protection of murine macrophages by sEntV68 during C. albicans infection. (A) RAW264.7 murine macrophages were incubated with C. albicans SC5314 for 4 h with or without 100 nM sEntV68. Macrophage killing was evaluated by using the LDH cell toxicity assay, and the percentage of killed macrophages was calculated. (B) C. albicans survival in murine macrophages was assessed by using the XTT cell viability assay. (*P < 0.05; **P < 0.01). (C) RAW264.7 cells were infected with C. albicans (HWP1p::GFP, ADH1p::mCherry) for 1 h ± peptide followed by fixation and visualized by fluorescence microscopy. The percentage of hyphal cells was scored after 2 h of coculture and is given in the left image for both conditions. At least 200 cells were counted per replicate, and the experiment was repeated three times.