Fig. S4.

Pellino1 and Pellino2 function redundantly in the IL-1 pathway in IL-1R* cells. (A) WT IL-1R* cells (lanes 1 and 2) and Pellino1/TRAF6 double-KO cells (lanes 3 and 4) reexpressing Flag-tagged WT TRAF6, TRAF6[L74H], or TRAF6[120–522] (lanes 5–14) were stimulated with IL-1β, and the extracts were immunoblotted with antibodies that recognize the active, phosphorylated (p) forms of TAK1, IKKα/β, JNK1/2, p38α, and p38γ, as well as antibodies that recognize all forms of TRAF6. Pellino1, and GAPDH. (B) As in A, except that Pellino2/TRAF6 double-KO cells were used instead of Pellino1/TRAF6 double-KO cells. * denotes a nonspecific band detected by the antibody.