Fig. S2.

Sequence comparison of WT Shanqui Red (LGS1) and mutant alleles from Tetron (lgs1-5) and SC103 (lgs1-4) with the sorghum reference genome, BTx623 for the reverse strand of Sobic.005G213600. Genomic sequences of Shanqui Red and the mutants are from consensus of Illumina reads. Variations from the reference DNA sequence are indicated by bases in green, purple, and red for Shanqui Red, Tetron, and SC103, respectively. The predicted WT LGS1 protein is shown in green in three-letter residue code above codons. The predicted variations in mutant peptides are shown in purple and red for Tetron and SC103, respectively. Residues highlighted in yellow are PAPS binding regions that are broadly conserved in sulfotransferases of all organisms. Chromosome positions according to Phytozome Sorghum bicolor v3.1, DOE-JGI.