Table S3.

Summary of deletion variants in the LGS1 region

Sorghum accession	Allele name	Deletion position* Chr05:	Sobic.005G213300†	Sobic.005G213400	Sobic.005G213500	Sobic.005G213600	Sobic.005G213700	Sobic.005G213766	Sobic.005G213832	Sobic.005G213900
			No annotated functional domains	No annotated functional domains	2OG-Fe(II) oxygenase superfamily; nonhaem dioxygenase in morphine synthesis N terminal	Weakly similar to Os09g0555100 protein (sulfotransferase family)	Similar to epoxide hydrolase (alpha/beta hydrolase fold)	No annotated functional domains	UDP-glucose–glucose-phosphate glucosyltransferase	Gamma-thionin family
SRN39	lgs1-1	69,977,147..70,011,172	Intact	Intact	Deleted	Deleted	Deleted	Deleted	Deleted	Intact
555	lgs1-2	69,958,403..69,986,951	Intact	Deleted	Deleted	Deleted	Intact	Intact	Intact	Intact
IS7777	lgs1-3	69,981,523..70,011,172	Intact	Intact	Intact	Deleted	Deleted	Deleted	Deleted	Intact
SC103	lgs1-4	69,985,178..69,985,598	Intact	Intact	Intact	421-bp deletion; premature stop	Intact	Intact	Intact	Intact
Tetron	lgs1-5	69,985,617..69,985,626	Intact	Intact	Intact	10-bp deletion; premature stop	Intact	Intact	Intact	Intact

^*Phytozome, Sorghum bicolor v3.1 DOE-JGI.

^†Gene model and annotation.