Fig. 1.

Low-Pi insensitive mutants continue primary root growth and RAM maintenance under Pi-deficiency conditions. (A) Phenotypes of WT, lpi5, and lpi6 seedlings grown under high (1,000 μM Pi; +Pi)- and low-Pi (5 μM Pi; −Pi) conditions 10 dag. (Scale bar, 1 cm.) (B) Primary root growth kinetics of seedlings grown under −Pi conditions from 2 to 10 dag. Green, blue, and red dots depict WT, lpi5, and lpi6 individuals (n = 30 from three independent experiments), respectively. Statistical groups were determined using a Tukey honest significant difference (HSD) test (P value <0.05) and are indicated with letters. (C) Primary root length of seedlings grown under +Pi and −Pi conditions. Green, blue, and red dots depict WT, lpi5, and lpi6 individuals (n = 30 from three independent experiments), respectively. Statistical groups were determined using a Tukey HSD test (P value <0.05) and are indicated with letters. (D) GUS staining of proCycB1::GUS and proQC46::GUS expression in the RAM of WT, lpi5, and lpi6 seedlings 10 dag. (Scale bar, 100 µm.)