Fig. 4.

Malate treatment rescues the mutant phenotype of stop1 and almt1 seedlings. (A) Primary root length of 10 dag WT, stop1, and almt1 seedlings grown under increasing concentration of malate supplemented into −Pi medium. Green, blue, and red dots depict WT, stop1, and almt1 individuals (n = 30 from three independent experiments), respectively. Statistical groups were determined using Tukey HSD test (P value <0.05) and are indicated with letters. (B) Phenotypes of 10 dag WT, stop1, and almt1 seedlings grown under low-phosphate medium (−Pi) and low-phosphate medium supplemented with 1 mM malate (−Pi/+malate). (Scale bar, 1 mm.) (C) proCycB1::GUS (Lower) and proQC46::GUS (Upper) expression. (D) Perls-DAB iron staining in the RAM of WT, stop1, and almt1 seedlings grown under −Pi and malate treatment (1 mM; −Pi/+malate) conditions 5 dag. Pictures show photographic reconstructions in which several images were spliced together to show the complete primary root meristem (Materials and Methods). (Scale bar, 50 µm.)