Fig. 1.

Automated glycan assembly illustrated for the syntheses of oligosaccharides 12–14. (A) Schematic overview of AGA. (B) Detailed description of the full workflow demonstrated for the synthesis of oligosaccharide 12. Preautomation phase: preparation of building blocks, resin with linker and the synthesizer. Automation phase: AGA using the Glyconeer 2.1 synthesizer. Postautomation phase: analysis and purification of fully protected oligosaccharide 9, procurement of the conjugation-ready oligosaccharide 12, and quality control using IM-MS. (C) Protected oligosaccharides 10 and 11 are synthesized by selecting from a set of building blocks. AGA protocols and postautomation steps are performed to yield the conjugation-ready oligosaccharides 13 and 14.