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. 2017 Apr 10;114(17):4400–4405. doi: 10.1073/pnas.1703066114

Fig. 2.

Fig. 2.

Excision of N7-methylguanine by AlkZ. (A) Base excision of d7mG by AlkZ. (B) Denaturing PAGE of d7mG-DNA substrate (S) and nicked abasic-DNA product (P) after treatment without (−) or with (+) AlkZ for the specified time, followed by alkaline hydrolysis. (C) Quantitation of the gel shown in B, averaged across three independent experiments. Error bars represent SD. (D) Denaturing PAGE of d7mG-DNA substrate (S) and nicked abasic-DNA product (P) after treatment without (−) or with (+) AlkZ for 24 h, followed by treatment with water, NaOH, or EndoIV.