Fig. 5.

Functional importance of the IntS9–IntS11 interactions for snRNA 3′-end processing. (A) Schematic of the U7-GFP reporter that is transfected into human cells. (B) Western blot analysis of lysates from HeLa cells transfected with either control siRNA or IntS9 siRNA that then were transfected with either empty vector or myc-tagged RNAi-resistant IntS9. All cells were also transfected with the U7-GFP reporter. (C) The same analysis as in B, except that cells were treated with siRNA targeting IntS11 rather than IntS9. (D) Quantitative RT-PCR analysis of misprocessed U2 or U4 snRNA that are endogenously expressed. The bar graph represents the fold increase in the levels of misprocessed snRNA; data show the results of biological triplicates; error bars represent the SD from the mean.