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Endothelial deletion of S1pr1 did not affect subcellular distribution of CD31 and VE-cadherin. Representative immunoblot images of CD31 and VE-cadherin in each subcellular fraction from control (S1pr1flox/flox) and S1pr1iECKO brain microvessels are shown. Note that CD31 or VE-cadherin is exclusively detected in cell membrane or actin cytoskeletal fractions, respectively.
