Table 1.
Validation of the priming effect of the TLR2/6 ligand Pam2CSK4.
| Gene | Priminga | E. coli induction post primingb |
|---|---|---|
| TNF | 10.2 ± 3.0c | –1.3 ± 0.1 |
| IL1Β | 6.9 ± 1.8 | –1.6 ± 0.1 |
| IL6 | 25.2 ± 7.3 | 1.7 ± 0.4 |
| CXCL8 | 6.0 ± 0.7 | –1.2 ± 0.1 |
| NOS2A | 3.5 ± 0.5 | –1.7 ± 0.2 |
| TGM3 | 3.2 ± 0.7 | 3.9 ± 0.7 |
| SLPI | 10.8 ± 1.8 | 6.1 ± 1.4 |
| SAA3 | 204.6 ± 106.7 | 3.7 ± 1.1 |
| LAP | 4.3 ± 0.2 | 2.7 ± 0.3 |
a
Priming was with 1000 ng/ml of Pam2CSK4 for 12 h. bE. coli induction post-priming was for 3 h (see Figure 1), both treatments were applied in the same experimental setting as explained in Figure 2. Gray underlay, significant change (P < 0.05, Wilcox signed rank test). cValues are fold changes of mRNA concentrations relative to unprimed control cultures (means ± SEM from three different biological replica cultures, each assayed in duplicate).