Figure 2.

Study design for examining compounds in PubChem tested with AlphaScreen assay technology. Six assays targeting PPIs and using AlphaScreen were identified in PubChem. Only those compounds that were tested in all six assays were considered. Compounds were binned into two categories according to the number of active calls. Compounds in each category were then queried for PAINS alerts. The PubChem-wide bioassay activity of all compounds was then investigated.