Fig 5. ROC curve and AUC values from the simulated data with 4 and 6 replicates in each condition.

Illustrated are the ROC curves and AUC values (inset) in analyzing the impact of biological replicates on the performance of normalization methods. We used the simulated data with four biological replicates (A) and six biological replicates (B), which contain 1,500 DEGs with 2 FC difference between two conditions. Our methods (UQ-pgQ2 and Med-pgQ2) are in cyan and blue, respectively.