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. 2017 May 1;12(5):e0176184. doi: 10.1371/journal.pone.0176184

Fig 2. Plasmid constructs for protein-tagging.

Fig 2

The original plasmid is pBluescriptII SK, and the maps display the restriction enzyme sites used for construction. (A) The pCUC map contains the 5’ mChe-URA3-3’ erry cassette. (B) The psfGUG map contains the 5’ sfG-URA3-3’ GFP cassette. (C) The pKUK map contains the 5’ mKik-URA3-3’ kGR cassette. For pCUC, psfGUG and pKUK, each pair of sectional tag sequences has a partially duplicated sequence for homologous recombination. (D) The pHUH map contains the 5’HA-URA3-3’HA cassette. (E) The pFUF map contains the 5’3×FLAG-URA3-3’3×FLAG cassette. For these two plasmids, the HA and FLAG tags are too short to be split, so the full-length sequences are used for homologous recombination.