Skip to main content
. 2017 May 1;12(5):e0176184. doi: 10.1371/journal.pone.0176184

Fig 7. PCR and western blot analysis of HA-tagged Ubc13 yeast strains.

Fig 7

(A) Ubc13-HA strain. (B) HA-Ubc13 strain. Lane 1: DNA ladder marker. Lanes 2 and 4: the original strain BY4741. Lane 3: the pop-in strain. Lane 5: the pop-out strain. Arrows indicate the locations of primers. The detail information of primers could be found in the part of yeast strains check primers in S1 Table. And the strains in each Lane 4 and 5 share the same pair of primers for analysis. (C) (Upper) Anti-HA antibody is used for detecting the HA tag. (Bottom) Ponceau S staining is shown as the total protein loading control.