Table 2. Proteins tagging primers used in this studyɑ.
| Primers | Sequences (5’→3’) |
|---|---|
| HA-Ubc13 | |
| pNUbc13-HUH F |
TTAGCAAATAAGGTCAGGTTCATTGTAACATAGTTAGAA ATGTACCCATACGATGTTCC |
| pNHUH-Ubc13 R |
TGAACATACCTTGATTATTCTCTTGGGTAATGATGCCAT AGCGTAATCTGGAACATCGT |
| UBC13-HA | |
| pCUbc13-HUH F |
CGCGAATGGACGAAATTGTATGCAAAGAAGAAACCCGAG ATGTACCCATACGATGTTCC |
| pCHUH-Ubc13 R |
TTATATATTCAGTTGAGAAAACTTATACAGAAATGATCA AGCGTAATCTGGAACATCGT |
| 3xFLAG-Ubc13 | |
| pUbc13-FUF F |
TTAGCAAATAAGGTCAGGTTCATTGTAACATAGTTAGAA ATGGACTACAAAGACCATGA |
| pFUF-Ubc13 R |
TGAACATACCTTGATTATTCTCTTGGGTAATGATGCCAT CTTGTCATCGTCATCCTTGT |
| mCherry-Ubc13 | |
| pUbc13 F pUbc13-mCherry R pUbc13-mCherry F |
AGCTTAATGATTAGATTCTG TCCTCGCCCTTGCTCACCATTTCTAACTATGTTACAATGA TCATTGTAACATAGTTAGAAATGGTGAGCAAGGGCGAGGA |
| pmCherry-Ubc13 R pmCherry-Ubc13 F |
CTCTTGGGTAATGATGCCATCTTGTACAGCTCGTCCATGC GCATGGACGAGCTGTACAAGATGGCATCATTACCCAAGAG |
| pUbc13 R Rad5-sfGFP pRad5 F pRad5-sfGFP R |
AAGTAACGTAAGTTGTCGTC GCCGTTAAAGACTATAGCAG AGCTCTTCGCCTTTACGCATTTCAAACAGCATCTGGATTTC |
| pRad5-sfGFP F psfGFP-Rad5 R psfGFP-Rad5 F |
GAAATCCAGATGCTGTTTGAAATGCGTAAAGGCGAAGAGCT TATGAGTATGTGGTATGACTA TCATCATTTGTACAGTTCATCCATA TATGGATGAACTGTACAAATGATGATAGTCATACCACATACTCATA |
| pRad5 R |
CCACCTACACTTTCTGTCAT |
| Rad5-mKikGR | |
| pRad5-mKikGR F pRad5-mKikGR R pmKikGR-Rad5 F |
GAAATCCAGATGCTGTTTGAA ATGAGTGTGATTACATCAGA TCTGATGTAATCACACTCATTTCAAACAGCATCTGGATTTC GGGCGCCAAGTATGAATTTGAAGCCTAGTCATACCACATACTCATA |
| pmKikGR-Rad5 R |
TATGAGTATGTGGTATGACTA GGCTTCAAATTCATACTTGGCGCCC |
| HHT1-sfGFP | |
| pHHT1-sfGFP F | GATATCAAGTTGGCTAGAAGATTAAGAGGTGAAAGATCA ATGCGTAAAGGCGAAGAGCT |
| pHHT1-sfGFP R |
GTTCGTTTTTTACTAAAACTGATGACAATCAACAAACTA TCATCATTTGTACAGTTCATCCATA |
ɑ Primers used to amplify each cassette are listed here. Each primer consists of two parts. One part is the sequence corresponding to each cassette element. Another part underlined in the list is the sequence complementary to the gene-specific sequence.