Table 3. Differential expression of novel and conserved tae-miRNAs in wheat seeds.
| NO. | Mature miRNA |
Sequence(5'-3') | Length (nt) |
Log2 (T349/J19) |
Log2 (L21/J19) |
Log2 (J22/J19) |
RPM in each bank | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T349 | J19 | J22 | L21 | ||||||||||||||||||
| 1 | tae-N1 | GCCTCCGTAGCATAGTGGT | 19 | 1.89 | ↑ | -0.12 | — | -0.10 | — | 10.197 | 4.846 | 16.762 | 3.99 | 2.441 | 3.02 | 4.985 | 3.048 | 0.762 | 1.648 | 1.95 | 3.086 |
| 2 | tae-N2 | GCGTCTGTAGTCCAACGGT | 19 | 1.32 | ↑ | -0.01 | — | -0.38 | — | 838.611 | 575.006 | 801.28 | 379.006 | 285.187 | 354.115 | 315.519 | 270.286 | 192.824 | 241.649 | 242.087 | 298.066 |
| 3 | tae-N3a, b | ATTTGTAGTGTTCGGATTGAGTTT | 24 | 1.23 | ↑ | 0.92 | — | 1.18 | ↑ | 10.872 | 14.935 | 12.319 | 7.102 | 6.73 | 4.706 | 15.731 | 14.718 | 11.65 | 8.456 | 7.36 | 11.364 |
| 4 | tae-N4a, b, c, d, e, f, g | GTGGCTGTAGTTTAGTGG | 18 | 1.29 | ↑ | 2.50 | ↑ | -2.00 | ↓ | 11.302 | 22.164 | 9.895 | 14.881 | 9.237 | 15.665 | 9.306 | 9.566 | 6.423 | 6.736 | 5.095 | 4.7 |
| 5 | tae-N5 | GGGGGTGTAGCTCATATGGT | 20 | 1.65 | ↑ | -0.36 | — | -1.80 | ↓ | 18.489 | 23.038 | 19.185 | 23.742 | 27.713 | 19.177 | 37.224 | 38.687 | 33.099 | 16.052 | 15.728 | 17.256 |
| 6 | tae-N6 | GTTTGACTTTGCACTGCTAGCGGC | 24 | 1.21 | ↑ | -2.27 | ↓ | 3.15 | ↑ | 2.088 | 1.668 | 3.433 | 6.29 | 5.74 | 7.656 | 0.221 | 1.471 | 1.85 | 10.534 | 9.499 | 10.031 |
| 7 | tae-N7 | TTTTTTGATCCTTAGGATGGC | 21 | 1.15 | ↑ | -0.06 | — | 1.26 | ↑ | 3.378 | 3.098 | 3.567 | 1.623 | 1.979 | 1.334 | 15.066 | 16.189 | 13.392 | 0.286 | 0.314 | 0.07 |
| 8 | tae-N8 | CTTGTTTGTCATTAAGCTTCTG | 22 | 1.16 | ↑ | -1.34 | ↓ | -1.38 | ↓ | 6.204 | 6.673 | 7.135 | 7.778 | 12.141 | 9.202 | 4.431 | 5.992 | 3.919 | 4.944 | 6.291 | 5.471 |
| 9 | tae-N9 | GCTGGAGTAGCTCAGTTGGT | 20 | 1.47 | ↑ | -0.13 | — | -2.13 | ↓ | 14.435 | 13.902 | 10.77 | 6.29 | 6.928 | 7.024 | 15.731 | 16.189 | 16.549 | 4.156 | 5.284 | 5.471 |
| 10 | tae-N10 | GCGGGAGGCACGGGGTTCG | 19 | -1.22 | ↓ | 0.97 | — | -2.37 | ↓ | 24.878 | 34.16 | 27.061 | 14.949 | 16.298 | 12.714 | 7.311 | 4.625 | 4.681 | 4.228 | 4.718 | 4.279 |
| 11 | tae-N11a, b, c | ATCAGAGTGGCGCAGCGGA | 19 | 1.15 | ↑ | 0.02 | — | -2.36 | ↓ | 26.291 | 24.865 | 27.802 | 11.972 | 9.831 | 10.818 | 3.434 | 1.366 | 2.395 | 7.381 | 8.052 | 7.506 |
| 12 | tae-miR396c-N12a, b | TCCACAGCTTTCTTGAACTG | 20 | 1.24 | ↑ | 1.03 | ↑ | 1.25 | ↑ | 6.941 | 10.565 | 5.789 | 3.652 | 2.837 | 4.636 | 9.084 | 9.777 | 7.839 | 4.371 | 5.85 | 7.576 |
| 13 | tae-miR164c-N13 | TGGAGAAGCAGGGCACGTGCT | 21 | 1.38 | ↑ | 0.32 | — | 1.62 | ↑ | 7.739 | 6.355 | 8.078 | 3.314 | 2.969 | 3.442 | 9.084 | 9.251 | 11.541 | 2.938 | 3.271 | 3.156 |
| 14 | tae-miR827-N14 | TTAGATGACCATCAGCAAACA | 21 | 1.24 | ↑ | 0.00 | — | 1.39 | ↑ | 6.941 | 7.547 | 7.404 | 4.193 | 2.969 | 3.371 | 9.416 | 5.992 | 12.085 | 3.368 | 2.327 | 2.455 |
| 15 | tae-miR396g-N15 | TCCACAGGCTTTCTTGAACTG | 21 | 1.00 | ↑ | 0.54 | — | 1.13 | ↑ | 101.97 | 101.845 | 90.676 | 58.308 | 56.681 | 54.23 | 129.62 | 131.936 | 108.552 | 56.9 | 60.396 | 72.254 |
| 16 | tae-miR166a-N16 | TCGGACCAGGCTTCATTCC | 19 | 1.12 | ↑ | 1.38 | ↑ | 1.20 | ↑ | 48.527 | 57.357 | 46.314 | 29.627 | 35.236 | 30.416 | 88.739 | 87.046 | 63.585 | 39.056 | 36.363 | 54.786 |
↑, up-regulated; ↓, down-regulated;—, no significant difference. No.1-11, novel miRNAs; No. 12–16, conserved miRNAs.