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. 2017 Mar 29;174(1):223–240. doi: 10.1104/pp.16.01282

Figure 3.

Figure 3.

Morphology of exo70C2 pollen tubes germinated in vitro. A to C, Germinated pollen of a wild-type plant (A), an exo70C2 heterozygous plant (B), and an exo70C2 homozygous plant (C) in the quartet-1 background. Putative wild-type or mutant pollen tubes are marked by white or black arrows, respectively; arrows of the same design correspond to the same tetrad. Bars = 100 μm. D, Distribution of pollen tube lengths in the samples above (A–C). E, A typical wild-type pollen tube. Bar = 20 μm. F to K, Mutant pollen tubes showing relatively normal morphology (F), branching (G and I), sharp bending (H), effusion of cytoplasm (G and I; marked by arrowheads), or the production of protoplast-like structures emerging from tube tips (J and K; marked by asterisks). Bars = 20 μm. L, Aniline Blue staining of pollinated pistils of the wild type (WT) and an exo70C2 homozygote visualizing callose in pollen tubes. Bar = 200 μm.