Figure 4.

DGAT activity in extracts of developing seeds from transgenic camelina plants expressing CpuDGAT1. Measurement of DGAT activity in crude extracts from developing wild-type camelina seeds (22 DAF) and developing camelina seeds expressing CvFatB1 alone, coexpressing CvFatB1 and CpuDGAT1 (CvFatB1+CpuDGAT1), or coexpressing CvFatB1, CvLPAT2, and CpuDGAT1 (CvFatB1+CvLPAT2+CpuDGAT1). Results are for assays using [1-¹⁴C] 10:0-CoA and DAG species: 10:0/10:0 and 18:1/18:1, respectively. Values are mean ± sd (n = 3). Asterisks denote statistically significant differences (*P < 0.0001) as compared to wild type, as determined by two-tailed Student’s t test. Wt, wild type.