Figure 9.

Seed weight, germination efficiency, and total fatty acid content of wild-type camelina seeds and seeds of engineered camelina lines expressing CvFatB1 alone or in combinations with CvLPAT2 and CpuDGAT1. Shown are 100-seed weight (A), germination efficiency (B), and total fatty acid content (C) of mature seeds from wild-type camelina and camelina seeds engineered for expression of CvFatB1 alone, with CvLPAT2 (CvFatB1+CvLPAT2) or CpuDGAT1 (CvFatB1+CpuDGAT1), and with the combination of CvLPAT2 and CpuDGAT1 (CvFatB1+CvLPAT2+ CpuDGAT1). Values shown for 100-seed weight are the means and ± sd of the mean for four biological replicates. Values for seed fatty acid content are the means and ± sd of the mean for three biological replicates. Asterisks indicate statistically significant difference (*P < 0.005, **P < 0.025) as compared to the wild type based on two-tailed Student’s t test. Values for germination efficiency are the means and ± sd of the mean for three biological replicates. Asterisks denote statistically significant difference (*P < 0.005, ***P < 0.05) in germination efficiency of transgenic seeds as compared to the CvFatB1+ CvLPAT2+CpuDGAT1 seeds based on two-tailed Student's t test. Wt, wild type.