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. 2017 Mar 27;174(1):154–171. doi: 10.1104/pp.16.01962

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Figure 5. — Yeast two-hybrid assay demonstrating the direct interaction of MYB134 and MYB115 with bHLH131. The conserved N-terminal regions of MYB134 and MYB115 were fused to the binding domain (BD) and bHLH131 was fused to the activation domain (AD) of the vector. A, The interaction was tested in the yeast two-hybrid assay using two selectable markers, the His/Ade auxotrophs (middle panel) and Aureobasidin A (AbA; right panel). Control colonies with no selection are shown on the left panel. When fused to the BD vector, MYB134, MYB115, and bHLH131 all have autoactivation (data not shown). B, Serial dilution (5-fold dilutions) of the two-hybrid assay with AbA.