Table II. Induction of flavonoid and MYB transcripts by leaf wounding in P. tremula × P. tremuloides saplings.
Transcript | Relative Expression (ΔCt) |
Fold Changeb | Pc | |
---|---|---|---|---|
Control | Woundeda | |||
DFR1 | 0.20 | 1.64 | 8.36 | 0.09 |
ANR1 | 0.68 | 4.02 | 5.90 | 0.07 |
MYB134 | 2.55 | 8.40 | 3.29** | 0.02 |
MYB115 | 0.07 | 0.33 | 4.50** | 0.03 |
Leaf margins were wounded by crushing with pliers 24 h prior to harvest. Leaf plastochron index (LPI) 10 to 12 leaves were collected from each plant and pooled for analysis.
Relative transcript levels in wounded versus control leaves. Boldface values and asterisks indicate significance at the threshold of P ≤ 0.05 (n = 3).
P values indicate results of a one-way ANOVA.