Figure 6.

pTAC10 interacts with PAPs. The expression patterns of PAPs in the ptac10-1 mutants and the interactions of pTAC10 with PAPs were analyzed. A, Heat map showing the expression pattern of 12 PAPs in the ptac10-1 mutants and wild-type Col-0 plants (WT). Expression patterns of these genes were collected from RNA sequencing results using 1-week-old Col-0 and ptac10-1 mutant plants. B, Yeast two-hybrid assay showing that pTAC10 interacts with subunits of the PEP complex. The yeast line cotransformed with the p53 bait and T prey plasmids was used for a positive control, and the yeast line transformed with the LAM bait and T prey plasmids was used for a negative control. The yeast lines transformed with blank prey plasmids together with pTAC10 bait plasmids were used for the test of self-activation. –Leu –Trp +Abs A indicates Abs A-containing double dropout (DDO) medium (–Leu –Trp) for the test of pTAC10 interaction. Blue boxes indicate the yeast lines that survived in the Abs A-containing medium. C, Blank bait plasmid analysis to verify the interaction of pTAC10-PAPs in the yeast two-hybrid assay. D, GST pull-down assay showing that pTAC10 interacts with the PAPs. MBP and GST proteins were used as negative controls for the pTAC10-PAP interaction. Interaction analyses were repeated twice with similar results.