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. 2017 Mar 23;174(1):435–449. doi: 10.1104/pp.17.00248

Figure 8.

Figure 8.

The pTAC10 C-terminal region downstream of the S1 domain mediates the pTAC10-PAP interactions. A, Schematic of the truncated pTAC10 protein structure. B and D, Yeast two-hybrid assay results using pTAC10-1245 (B), pTAC10-1467 (C), and pTAC10-1740 (D) as bait plasmids. Blue boxes indicate the yeast lines that survived in the Abs A-containing medium. E, Coimmunoprecipitation analysis of the pTAC10 and FSD3 interaction. pTAC10-HA and pTAC10ΔC-HA indicate intact pTAC10 and pTAC10 lacking their C-terminal regions downstream of the S1 region fused with the HA epitope, respectively. FSD3-myc indicates FSD3 proteins fused with the myc epitope. F, Morphology of the ptac10-1 mutant transformed with the 35s::pTAC10-1245, 35s::pTAC10-1467, or 35s::pTAC10-1740 plasmid. Introduction of the 35s::pTAC10-1245, 35s::pTAC10-1467, or 35s::pTAC10-1740 plasmid did not rescue the ptac10-1 mutant phenotype, leading to seedling lethality. Interaction analyses were repeated twice with similar results. Bars = 1 mm.