Figure 3.
The period of the circadian rhythm of photoaccumulation is lengthened in pcrymut. A to C, Chlamydomonas was grown in Tris-acetate-phosphate (TAP) medium under an LD12:12 cycle. Cells were then transferred to minimal medium and maintained in constant darkness for the indicated amount of time before photoaccumulation of cells in a light beam was quantified. The ordinate shows cell density documented as relative extinction in percentage, and the abscissa shows time in constant darkness in days. Photoaccumulation curves of the wild type (WT; A), pcrymut (B), and the complemented mutant (C) are shown. D, Mean values of the free-running periods (n ≥ 5) of the wild type, pcrymut, and the complemented mutant are shown in hours ± sd. Significant differences were estimated by Student’s t test: *, P < 0.05; n.s., not significant.