Figure 2.
Comparison of oral mycobiome members (genus-level) as identified by molecular and culture-based approaches. Most prevalent oral genera (appearing in ∼20% of subejcts in Ghannoum et al.4 and Dupuy et al.5) DNA sequencing studies are in the left circle. Those studies38,41,42,44 appear in the right circle. The overlap in the Venn diagram illustrates the fungal genera identified by both approaches. Highlighted genera in the culture-based list are those present in our saliva data set but at less than 20% frequency.5 The molecular studies were conducted on systemically healthy individuals, while culture-based studies included both healthy and cancer patients. Samples in the molecular studies included oral rinses and unstimulated saliva, while samples for the cultivation studies included oral rinses, mucosal swabs and contents of infected root canals. Despite these differences, there is great agreement between the molecular surveys and cultivation studies with most taxa detected by cultivation also seen molecularly.