| pGEM®-T ligation | 1 Reaction |
|---|---|
| Nuclease-free Water | 1 μl |
| 2X Rapid ligation buffer | 2.5 μl |
| pGEM®-T vector | 0.5 μl |
| DO-RIP-seq cDNA | 0.5 μl |
| T4 DNA ligase | 0.5 μl |
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