Skip to main content
. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Methods. 2016 Dec 18;118-119:50–59. doi: 10.1016/j.ymeth.2016.12.007
PCR_5_F: CGACGGCCAGTG - gene-specific primer
PCR_5_R: GGCTTACCGAATTC - gene-specific primer (starts at base before stop codon)
PCR_3_F: CTAGATCGGATCC - gene-specific primer (starts at base after stop codon)
PCR_3_R: GCATGCAGTCGA - gene-specific primer