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. 2017 May 2;8:738. doi: 10.3389/fmicb.2017.00738

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Copyright © 2017 Wampach, Heintz-Buschart, Hogan, Muller, Narayanasamy, Laczny, Hugerth, Bindl, Bottu, Andersson, de Beaufort and Wilmes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of prokaryotes, archaea and fungi in infant stool during the first year of life. (A) Absolute quantification of 16S rRNA gene copy numbers for prokaryotic DNA (ng DNA per mg of stool), (B) relative quantification of archaeal read counts. (C) Absolute quantification of 18S rRNA gene copy numbers for fungal DNA (ng DNA per mg of stool) by quantitative real-time PCR and over the course of the first year of life. The numbers of samples per collection time point are provided at the top of the graph. For the purpose of clarity, only significant differences between subsequent time points are shown in the figure; for all significant differences between collection time points, see Supplementary File 1, Tables S3, S4. Significant differences obtained by Wilcoxon rank sum test between consecutive time points are represented by asterisks (^* when P < 0.05; ^** when P < 0.01). CSD, C-section delivery; VD, vaginal delivery. Fecal samples originating from VD infants are represented on the left side of each barplot and by green points, samples from CSD infants are represented on the right side of each barplot and by blue points.