Table 1.
Immunophenotype analysis.
| Lymphocyte subsets | % | Absolute count × 10e9/l | Gated on | Reference values % Median (5th–95th percentile) | Reference values Absolute count × 10e9/l (5th–95th percentile) |
|---|---|---|---|---|---|
| CD3+/CD45+ | 89.1 | 2.3 | Lymphocytes | 67 (50–91) (25) | 1.5 (0.78–3.0) (25) |
| NK (CD3−/CD56+) | 6.0 | 0.15 | Lymphocytes | 15 (5–49) (25) | 0.34 (0.10–1.2) (25) |
| CD3+/CD4+ | 37 | 0.96 | Lymphocytes | 42 (28–64) (25) | 1.0 (0.5–2.0) (25) |
| CD3+/CD4+/CD27+CD45RA+Naive T helper | 65 | 0.62 | CD4+ | 46 (16–100) (25) | 0.5 (0.1–2.3) (25) |
| CD3+/CD4+/CD27+CD45RA−Central memory T helper | 23.7 | 0.23 | CD4+ | 42 (18–95) (25) | 0.43 (0.18–1.1) (25) |
| CD3+/CD4+/CD27−CD45RA−Effector memory T helper | 2.6 | 0.025 | CD4+ | 5 (1–23) (25) | 0.053 (0.013–0.22) (25) |
| CD3+/CD4+/CD27−CD45RA+Effector memory CD45RA+ T helper | 4.6 | 0.044 | CD4+ | 0.35 (0.0083–6.8) (25) | 0.0037 (0.000098–0.068) (25) |
| CD3+/CD4+/CD31+/CD45RA+Recent thymic emigrants | 48.4 | 0.46 | CD4+ | 33 (7–100) (25) | 0.34 (0.05–2.4) (25) |
| CD3+/CD8+ | 30.1 | 0.78 | Lymphocytes | 22 (12–40) (25) | 0.5 (0.2–1.2) (25) |
| CD3+/CD8+/CCR7+/CD45RA+Naive T cytotoxic | 35.0 | 0.27 | CD8+ | 29 (6–100) (25) | 0.13 (0.016–1.0) (25) |
| CD3+/CD8+/CCR7+/CD45RA−Central memory T cytotoxic | 2.3 | 0.02 | CD8+ | 5 (1–20) (25) | 0.024 (0.0047–0.12) (25) |
| CD3+/CD8+/CCR7−CD45RA−Effector memory T cytotoxic | 21.2 | 0.16 | CD8+ | 36 (14–98) (25) | 0.16 (0.04–0.64) (25) |
| CD3+/CD8+/CCR7−CD45RA+Effector memory CD45RA+T cytotoxic | 41.5 | 0.32 | CD8+ | 19 (7–53) (25) | 0.084 (0.025–0.28) (25) |
| TCR alpha/beta | 97 | 2.25 | CD3+ | 59 (36–98) (25) | 1.4 (0.6–3.3) (25) |
| TCR gamma/delta | 0.4 | 0.009 | CD3+ | 3 (0.83–11) (25) | 0.071 (0.025–0.2) (25) |
| CD4−CD8−(DN) TCR alpha/beta+ | 1.2 | 0.027 | TCR alpha/beta+ | 2 (0.57–5) (25) | 0.023 (0.0069–0.074) (25) |
| CD19+ | 3 | 0.078 | Lymphocytes | 15.2 (6.5–24.0) (25) | 0.34 (0.19–0.62) (25) |
| CD27+IgD+IgM+ | 15.3 | 0.012 | CD19+ | 6.4 (2.6–13.4) (26) | 0.020 (0.007–0.056) (26) |
| CD27+IgD−IgM− | 14.8 | 0.011 | CD19+ | 9.1 (4.0–21.2) (26) | 0.033 (0.012–0.069) (26) |
| CD27−IgD+IgM+ | 66.6 | 0.052 | CD19+ | 79.6 (61.6–87.4) (26) | 0.254 (0.126–0.546) (26) |
| CD27−IgD−IgM− | 3.2 | 0.002 | CD19+ | 4.9 (1.4–13.0) (26) | 0.016 (0.006–0.042) (26) |
| MEMORY CD19+CD27+ | 30.1 | 0.023 | CD19+ | 16.0 (7.0–29.0) (26) | 0.057 (0.026–0.115) |
| CD21LOW CD38 NEG | 13.4 | 0.010 | CD19+ | 4.4 (1.6–10.0) (27) | 0.01 (0.01–0.02) (27) |
| Transitional IgM++CD38++ | 2.22 | 0.002 | CD19+ | 2.5 (0.9–5.7) (27) | 0.01 (0.00–0.03) (27) |
Data are presented as representative relative numbers (%) and absolute counts of lymphocyte subsets.
The absolute size of each lymphocyte subpopulation was calculated by multiplying the relative size of the lymphocyte subpopulation and the absolute lymphocyte count.