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Transformation of E. coli strains that possess different RM systems. The X axis indicates the absence (R−M−) and presence of a Type I RM system, represented by restriction enzymes EcoKI (IA), EcoIA (IB), EcoR124I (IC), and StySBLI (ID), expressed by an E. coli strain. The Y axis denotes the number of unmethylated pHS10842 transformants. Data are the means ± SD derived from three similar experiments. *Significantly less than R−M−; P < 0.05.
