Table 1.
Primers used in this study.
| No | Primer name | Sequence (5′–3′) | Product size (bp) |
|---|---|---|---|
| P1 | KPC-2F | TGTAAGTTACCGCGCTGAGG | |
| P2 | KPC-2R | CCAGACGACGGCATAGTCAT | 584 |
| P3 | KlcAHSF | GGCTTATTGGCTTATGTGG | |
| P4 | KlcAHSR | GTAGAGGCAAGCGGTAAT | 145 |
| P5 | Site1F | CCTGGAGTACAGGCGATCGCCTTGCCCTGCTTCTGGAACTGCTG | |
| P6 | Site1R | GGGCAAGGCGATCGCCTGTACTCCAGGATCGGCCAGGCG | 13,935 |
| P7 | Site2F | CACAGGCGGTCGCGCTGGGTTCCTTCTTCTTGCCGCCAG | |
| P8 | Site2R | GAAGGAACCCAGCGCGACCGCCTGTGCAAGCAGCTCG | 13,928 |
| P9 | SX1F | TACAGGCGCACCTTGTCGTT | |
| P10 | SX1R | GCGAGGGTGGGCAGATGAC | 223 |
| P11 | SX2F | GCGGTCGAACACGTGTGTG | |
| P12 | SX2R | TCAATCCAGCCGTCTCGCG | 410 |
| P13 | CX1F | CGCGCAGTTCCAGCACCTCC | |
| P14 | CX1R | AAGACCTCGGTAAGTTCGAG | 420 |
| P15 | CX2F | CTTGACGTAATTGCGCAGCGT | |
| P16 | CX2R | TCATCGAGCTCTATGCCGAC | 341 |
| P17 | KlcAHS-F | ACGGTGTCATATGATGCAAACAGAACTTAA | |
| P18 | KlcAHS-R | GCTAGAATTCCTAGTCTATTGCGGCCAAG | 426 |
| P19 | T7primer F | TAATACGACTCACTATAGG | |
| P20 | T7terminator R | GCTAGTTATTGCTCAGCGG |
The underlined nucleotide sequences shown in P17 and P18 denote the NdeI and EcoRI recognition sites.