Table 2.
Strain/plasmid | Comment | Source |
---|---|---|
E. coli | ||
DH5α | Cloning strain | Novagen |
NM1049 | Type IA RM system, EcoKI, CmR | Prof. Dryden |
NK354 | Type IB RM system, EcoAI | Prof. Dryden |
NK402 | Type IC RM system, EcoR124I | Prof. Dryden |
NM1009 | Type ID RM system, StySBLI | Prof. Dryden |
NM1261 | RM system null, R−M− | Prof. Dryden |
PLASMID | ||
pHS10842 | Transformation AMPR, KP125892 | Our Lab |
pET24a | Transformation, KanR | Stratagene |