Fig. 5.

Feeding characteristics differ by behavioral state. A: pump rate was assessed before, immediately after, or 2 h after being picked from standard solid media with food into NGMB containing food. n = 5 worms. B: worms were immersed in NGMB with or without food and mounted on slides, where they incubated for 2 h. After 2 h worms on a given slide could be identified as swimming (S), dwelling (D), or quiescent (Q) based on locomotion. After finding and centering on worms their behavior was immediately recorded for 10 s at 100 Hz, and movies were reviewed at a later time to assess the pump rate. Number of worms observed in the indicated behavioral state for each condition from left to right: n = 9, 10, 2, 13, 13, and 3. n(fed) = 26; n(fasted) = 24. Error bars show SE.