FIG 3.

Time-related ethidium bromide fluorescence emission by the S. Senftenberg wild type (SSF WT) and the LASS strain. (a) The S. Senftenberg WT incubated with polymyxin B (PolyB) or linalool (Lin); (b) the LASS strain incubated with polymyxin B or linalool. Cells not incubated with polymyxin B or linalool served as controls. Fluorescence levels were determined during 50 min of incubation at 37°C. The results shown are the averages from 2 independent repeats performed in triplicate. Higher fluorescence values correlate with increased membrane permeability. Data for the wild type were published previously (16) and are provided here for the convenience of the reader.