TABLE 4.
Promoter | Ratio of expression by LASS/wild type with no inducerb | Fold induction compared with the basal level of expression withc: |
Ratio of expression by LASS/wild type with induction with 6 mM linaloold | |||
---|---|---|---|---|---|---|
5 mM decanoate |
6 mM linalool |
|||||
S. Senftenberg WT | LASS | S. Senftenberg WT | LASS | |||
marRAB | 5.2 ± 1.7* | 13.9 ± 3.2 | 5.6 ± 1.1* | 1.5 ± 0.5 | 1.2 ± 0.2* | 0.8 ± 0.3* |
acrAB | 1.7 ± 0.6* | 1.1 ± 0.8 | 1.8 ± 0.7* | 0.9 ± 0.4 | 1.0 ± 0.4 | 1.1 ± 0.7 |
micF | 7.2 ± 1.5* | 18.6 ± 4.0 | 4.1 ± 0.7* | 1.3 ± 0.2 | 1.4 ± 0.2 | 1.1 ± 0.2 |
rob | 1.6 ± 0.3* | 0.5 ± 0.2 | 1.2 ± 0.2* | 1.1 ± 0.3 | 1.0 ± 0.2 | 0.9 ± 0.3 |
soxS | 2.9 ± 0.8* | 1.6 ± 1.2 | 3.1 ± 0.9* | 1.1 ± 0.6 | 1.6 ± 0.5* | 1.4 ± 0.9* |
ramA | 0.5 ± 0.2* | 2.1 ± 0.7 | 3.5 ± 1.3* | 2.1 ± 0.7 | 1.9 ± 0.8 | 0.9 ± 0.5 |
mcpL | 43.5 ± 8.6* | 8.5 ± 4.8 | 1.5 ± 0.2* | 2.5 ± 1.3 | 1.0 ± 0.1* | 0.4 ± 0.2* |
Data were calculated from at least four experiments performed in triplicate. *, significant difference (P < 0.05) in promoter induction levels between the LASS strain and the S. Senftenberg wild type.
The numbers represent the average ratio of the fluorescence of the LASS mutant without an inducer normalized to the fluorescence of the same promoter without induction for the wild type. The basal promoter activities of the S. Senftenberg wild type represented by the normalized fluorescence are 725 for marRAB, 1,810 for acrAB, 5,938 for micF, 4,031 for rob, 388 for soxS, 2,467 for ramA, and 124 for mcpL.
The numbers represent the average ratio of the fluorescence of the promoter with induction normalized to the fluorescence of the basal level of the same promoter.
The numbers represent the average ratio of the fluorescence of the LASS mutant with induction with 6 mM linalool normalized to the fluorescence of the same promoter with induction with 6 mM linalool of the wild type.