FIG 4.
Replacement of Pad1 by AroY-B in detoxification of trans-cinnamic acid by Fdc1. (A) CEN.PK2-1C cells expressing Fdc1 alone, Pad1, or AroY-B together with Fdc1 or AroY-Ciso together with AroY-B and -D were spotted onto agar plates (SC, 2% glucose) supplemented with various concentrations of trans-cinnamic acid, as indicated. Cells transformed with empty vector were included as a control. The results were documented after 4 days of incubation at 30°C. (B) Growth phenotype of Pad1-expressing cells on different carbon sources. Cells expressing Pad1 or AroY-B with a C-terminal His tag were spotted onto agar plates with SC medium containing 2% glucose (SCD) or containing 2% glycerol and 2% ethanol (SCGE). Plates were incubated for 2 days (SCD plates) or 6 days (SCGE plates) at 30°C prior to documentation.