TABLE 1.
Plasmids used in this study
| Plasmid | Regulatory elements, markers, and/or genes | GenBank accession no. | Source or reference(s) |
|---|---|---|---|
| p426-MET25 | 2μ; MET25p; CYC1t; URA3 | 34, 38 | |
| p426-aroY-B-C-D | MET25p; aroY subunit B; CYC1t; PGK1p; aroY subunit C isoform; PGK1t; TPI1p; aroY subunit D; TAL1t; all subunits from K. pneumoniae codon optimized (DNA2.0 optimized) | AAY57854, AB479384, AAY57856 | This study |
| p426-aroY-PAD1-C-D | MET25p; PAD1 from strain S288C; CYC1t; PGK1p; aroY subunit C isoform; PGK1t; TPI1p; aroY subunit D; TAL1t | NP_010827, AB479384, AAY57856 | This study |
| p426-aroY-BMTS-C-D | MET25p; aroY subunit B with mitochondrial targeting signal of ATPase subunit 9 (amino acids 1 to 69); CYC1t; PGK1p; aroY subunit C isoform; PGK1t; TPI1p; aroY subunit D; TAL1t | CAA24230, AAY57854, AB479384, AAY57856 | This study |
| p426-aroY-PAD1Δ1–54-C-D | MET25p; PAD1 with an N-terminal deletion of amino acid residues 1 to 54; CYC1t; PGK1p; aroY subunit C isoform; PGK1t; TPI1p; aroY subunit D; TAL1t | NP_010827, AB479384, AAY57856 | This study |
| p426-AroY-B-C | MET25p; aroY subunit B; CYC1t; PGK1p; aroY subunit C isoform; PGK1t | AAY57854, AB479384 | This study |
| p426-FDC1 | TPI1p; FDC1 from strain S288C; TPI1t | NP_010828 | This study |
| p426-aroY-B-FDC1 | MET25p; aroY subunit B; CYC1t; TPI1p; FDC1; TPI1t | AAY57854, NP_010828 | This study |
| p426-PAD1-FDC1 | MET25p; PAD1; CYC1t; TPI1p; FDC1; TPI1t | NP_010827, NP_010828 | This study |
| p426-PAD1-His | MET25p; PAD1; CYC1t | NP_010827 | This study |
| p426-PAD1Δ1–54-His | MET25p; PAD1 with an N-terminal deletion of amino acid residues 1 to 54; CYC1t | NP_010827 | This study |
| p426-aroY-B-His | MET25p; aroY subunit B; CYC1t | AAY57854 | This study |
| p426-aroY-BMTS-His | MET25p; aroY subunit B with mitochondrial targeting signal of ATPase subunit 9; CYC1t | CAA24230, AAY57854 | This study |