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. 2017 May 1;83(10):e00035-17. doi: 10.1128/AEM.00035-17

FIG 1.

FIG 1

Natural amoebal isolate LPG3 requires both cyanobacterial and heterotrophic bacterial prey for optimal growth. (A) Fluorescence images of LPG3 cells showing ingested S. elongatus (Syn) cells, visible by chlorophyll autofluorescence. Scale bar, 10 μm. (B) Amoebal plaque formation on Syn lawns without antibiotics (left, BG11) and with antibiotics streptomycin and spectinomycin (right, SpSm) spotted with (1) LPG3 only, (2) LPG3 and Pseudomonas isolate LPH1 expressing aadA (Ps aadA), (3) LPG3 and wild-type Pseudomonas isolate LPH1 (wt Ps), (4) wt Ps only, and (5) Ps aadA only. (C to H) Amoebal growth curves and Pseudomonas CFU with Syn in BG11 medium with and without supplemented antibiotics (SpSm), with no added Pseudomonas (C, D), the addition of wild-type Pseudomonas (E, F), or Ps aadA (G, H). Values are averages of triplicate wells plus or minus standard errors of the mean (SEM). (I, J) Images showing Syn density at day 7 in BG11 (I) or BG11 with SpSm (J).